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primescripttm ii 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11022 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+1st+strand+cdna+synthesis+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 11022 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: The role of Pth1r in posterior cranium cartilage regulation and craniosynostosis.
    Article Snippet: .. Total RNA was isolated using Trizol (Ambion, Austin, TX, USA) and cDNA was subsequently synthesized using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio). .. The reaction mix was prepared using TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio) and was then subjected to real time PCR analysis using StepOne and StepOnePlus real time PCR systems (Life Technologies, Tokyo, JAPAN).

    Synthesized:

    Article Title: The role of Pth1r in posterior cranium cartilage regulation and craniosynostosis.
    Article Snippet: .. Total RNA was isolated using Trizol (Ambion, Austin, TX, USA) and cDNA was subsequently synthesized using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio). .. The reaction mix was prepared using TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio) and was then subjected to real time PCR analysis using StepOne and StepOnePlus real time PCR systems (Life Technologies, Tokyo, JAPAN).

    Article Title: The Effect of Heat Stress on Broiler Meat Quality and the Mechanisms Underlying Muscle Acidification: An In Vivo and In Vitro Study
    Article Snippet: .. RNA Extraction and RT-qPCR Total RNA was extracted from cultured DF-1 cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the PrimeScript® 1st Strand cDNA Synthesis Kit (Takara Bio Inc., Shiga, Japan). .. Real-time PCR was performed using BestarTM Real-time PCR Master Mix (DBI Bioscience, Dresden, Germany) on a CFX96 system (BioRad Laboratories, Hercules, CA, USA).

    cDNA Synthesis:

    Article Title: The role of Pth1r in posterior cranium cartilage regulation and craniosynostosis.
    Article Snippet: .. Total RNA was isolated using Trizol (Ambion, Austin, TX, USA) and cDNA was subsequently synthesized using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio). .. The reaction mix was prepared using TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio) and was then subjected to real time PCR analysis using StepOne and StepOnePlus real time PCR systems (Life Technologies, Tokyo, JAPAN).

    Article Title: Synthesis, structural characterization and molecular docking analysis of novel β-ketoiminato palladium( ii ) complexes with anticancer properties
    Article Snippet: Then, the total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen). .. The amount of 1000 ng of RNA was converted to complementary DNA (cDNA) using the PrimeScript 1st strand cDNA Synthesis Kit (Takara). .. Real-time PCR was performed using Applied BiosystemsTM PowerTrackTM SYBR Green Master Mix (ThermoFisher Scientific).

    Article Title: A molecular survey of orthohantaviruses in rodents across the tri-border region of China, Russia, and North Korea.
    Article Snippet: .. Detection and quantification of orthohantaviruses in rodent samples Viral RNA was extracted from the lung tissues using the TIANamp Virus RNA Kit (TIANGEN, China), and reversetranscribed into complementary DNA using the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa, Japan) according to the manufacturer’s instructions. ..

    Article Title: The Effect of Heat Stress on Broiler Meat Quality and the Mechanisms Underlying Muscle Acidification: An In Vivo and In Vitro Study
    Article Snippet: .. RNA Extraction and RT-qPCR Total RNA was extracted from cultured DF-1 cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the PrimeScript® 1st Strand cDNA Synthesis Kit (Takara Bio Inc., Shiga, Japan). .. Real-time PCR was performed using BestarTM Real-time PCR Master Mix (DBI Bioscience, Dresden, Germany) on a CFX96 system (BioRad Laboratories, Hercules, CA, USA).

    Article Title: Rapgef1 paralog-mediated regulation of Wnt/β-catenin signaling orchestrates early embryo tissue patterning and morphogenesis.
    Article Snippet: .. The total RNA was used to prepare cDNA using the PrimeScript 1st strand cDNA synthesis kit (TaKaRa, Cat. # 6110A). ..

    Article Title: Exon skipping by peptide nucleic acid derivatives
    Article Snippet: .. 200 ng of RNA template was subjected to a 25 μl reverse transcription reaction using PrimeScript 1st strand cDNA synthesis Kit (Cat. No. 6110B, Takara) against random hexamers. ..

    Article Title: Intracellular acid-base regulation mediates a trade-off between shell and somatic growth in a clam under ocean acidification
    Article Snippet: .. PrimeScript 1st strand cDNA synthesis kit , TaKaRa Biomedical Technology (Beijing) Co., Ltd. , 6110A. .. SMARTer® RACE 5’/3′ kit , Clontech , 634860.

    Article Title: Cathepsin A deficiency exacerbates LPS-induced inflammatory liver injury and apoptosis
    Article Snippet: Total RNA was isolated from spleen and liver tissue using the QIAzol lysis reagent (QIAGEN). .. RNA was reverse transcribed into cDNA using the PrimeScript 1st strand cDNA synthesis kit (TAKARA). .. Quantitative real-time PCR was performed using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (TAKARA) on a LightCycler 96 system.

    Virus:

    Article Title: A molecular survey of orthohantaviruses in rodents across the tri-border region of China, Russia, and North Korea.
    Article Snippet: .. Detection and quantification of orthohantaviruses in rodent samples Viral RNA was extracted from the lung tissues using the TIANamp Virus RNA Kit (TIANGEN, China), and reversetranscribed into complementary DNA using the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa, Japan) according to the manufacturer’s instructions. ..

    RNA Extraction:

    Article Title: The Effect of Heat Stress on Broiler Meat Quality and the Mechanisms Underlying Muscle Acidification: An In Vivo and In Vitro Study
    Article Snippet: .. RNA Extraction and RT-qPCR Total RNA was extracted from cultured DF-1 cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the PrimeScript® 1st Strand cDNA Synthesis Kit (Takara Bio Inc., Shiga, Japan). .. Real-time PCR was performed using BestarTM Real-time PCR Master Mix (DBI Bioscience, Dresden, Germany) on a CFX96 system (BioRad Laboratories, Hercules, CA, USA).

    Quantitative RT-PCR:

    Article Title: The Effect of Heat Stress on Broiler Meat Quality and the Mechanisms Underlying Muscle Acidification: An In Vivo and In Vitro Study
    Article Snippet: .. RNA Extraction and RT-qPCR Total RNA was extracted from cultured DF-1 cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the PrimeScript® 1st Strand cDNA Synthesis Kit (Takara Bio Inc., Shiga, Japan). .. Real-time PCR was performed using BestarTM Real-time PCR Master Mix (DBI Bioscience, Dresden, Germany) on a CFX96 system (BioRad Laboratories, Hercules, CA, USA).

    Cell Culture:

    Article Title: The Effect of Heat Stress on Broiler Meat Quality and the Mechanisms Underlying Muscle Acidification: An In Vivo and In Vitro Study
    Article Snippet: .. RNA Extraction and RT-qPCR Total RNA was extracted from cultured DF-1 cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the PrimeScript® 1st Strand cDNA Synthesis Kit (Takara Bio Inc., Shiga, Japan). .. Real-time PCR was performed using BestarTM Real-time PCR Master Mix (DBI Bioscience, Dresden, Germany) on a CFX96 system (BioRad Laboratories, Hercules, CA, USA).

    Reverse Transcription:

    Article Title: Exon skipping by peptide nucleic acid derivatives
    Article Snippet: .. 200 ng of RNA template was subjected to a 25 μl reverse transcription reaction using PrimeScript 1st strand cDNA synthesis Kit (Cat. No. 6110B, Takara) against random hexamers. ..

    Article Title: Cathepsin A deficiency exacerbates LPS-induced inflammatory liver injury and apoptosis
    Article Snippet: Total RNA was isolated from spleen and liver tissue using the QIAzol lysis reagent (QIAGEN). .. RNA was reverse transcribed into cDNA using the PrimeScript 1st strand cDNA synthesis kit (TAKARA). .. Quantitative real-time PCR was performed using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (TAKARA) on a LightCycler 96 system.



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